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dynamin related protein 1 drp1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc dynamin related protein 1 drp1
    Dynamin Related Protein 1 Drp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamin+related+protein+1/pm41843713-133-20-28
    Average 86 stars, based on 1 article reviews
    dynamin related protein 1 drp1 - by Bioz Stars, 2026-09
    86/100 stars

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    SDS Page:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Membrane:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Staining:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Incubation:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: α‑ketoglutarate protects against septic cardiomyopathy by improving mitochondrial mitophagy and fission
    Article Snippet: .. This was followed by an overnight incubation at 4°C with primary antibodies against atrial natriuretic protein (ANP; cat. no. ab225844; 1:500; Abcam), brain natriuretic peptide (BNP; cat. no. ab239510; 1:1,000; Abcam), β-major histocompatibility complex (β-MHC; cat. no. ab172967; 1:1,000; Abcam), BCL2 interacting protein 3 (Bnip3; cat. no. ab10433; 1:1,000; Abcam), LC3 (cat. no. 4599S; 1:1,000; Cell Signaling Technology, Inc.), Fis1 (cat. no. ab71498; 1:1,000; Abcam), dynamin-related protein 1 (DRP1; cat. no. 8570S; 1:1,000; Cell Signaling Technology, Inc.), NADH dehydrogenase (ubiquinone) 1α subcomplex subunit 12 (Ndufa12; cat. no. ab192617; 1:4,000; Abcam), NADH:ubiquinone oxidoreductase subunit AB1 (Ndufab1; cat. no. ab181021; 1:1,000; Abcam), mitochondrial NADH-ubiquinone oxidoreductase chain 1 (MT-ND1; cat. no. ab181848; 1:5,000; Abcam), succinate dehydrogenase (SDHA; cat. no. 11998; 1:4,000; Cell Signaling Technology, Inc.), complex IV (COX IV; cat. no. 11242-1-AP; 1:3,000; Proteintech Group, Inc.), oxoglutarate dehydrogenase (OGDH; cat. no. ab137773; 1:5,000; Abcam), pyruvate dehydrogenase (PDH; cat. no. 3205; 1:3,000; Cell Signaling Technology, Inc.), hypoxia inducible factor-1α (HIF-1α; cat. no. 36169; 1:1,000; Cell Signaling Technology, Inc.), NADPH oxidase 2 (NOX2; cat. no. bs-3889R; 1:1,000; BIOSS), NOX4 (cat. no. bs-1091R; 1:1,000; BIOSS), Bax (cat. no. 2772S; 1:3,000; Cell Signaling Technology, Inc.) and Bcl-2 (cat. no. 3498S; 1:1,000; Cell Signaling Technology, Inc.), GAPDH (cat. no. 10494-1-AP; 1:8,000; Proteintech Group, Inc.), α-tubulin (cat. no. 11224-1-AP; 1:4,000; Proteintech Group, Inc.) and HSP90 (cat. no. 11224-1-AP; 1:2,000; Proteintech Group, Inc.). .. Following incubation with the primary antibody, the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (anti-Mouse secondary antibodies; cat. no. 62-6520; 1:10,000; Thermo Fisher Scientific, Inc. and anti-Rabbit secondary antibodies; cat. no. VJ313046; 1:10,000; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

    Western Blot:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Binding Assay:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Aqueous Normal-phase Chromatography:

    Article Title: α‑ketoglutarate protects against septic cardiomyopathy by improving mitochondrial mitophagy and fission
    Article Snippet: .. This was followed by an overnight incubation at 4°C with primary antibodies against atrial natriuretic protein (ANP; cat. no. ab225844; 1:500; Abcam), brain natriuretic peptide (BNP; cat. no. ab239510; 1:1,000; Abcam), β-major histocompatibility complex (β-MHC; cat. no. ab172967; 1:1,000; Abcam), BCL2 interacting protein 3 (Bnip3; cat. no. ab10433; 1:1,000; Abcam), LC3 (cat. no. 4599S; 1:1,000; Cell Signaling Technology, Inc.), Fis1 (cat. no. ab71498; 1:1,000; Abcam), dynamin-related protein 1 (DRP1; cat. no. 8570S; 1:1,000; Cell Signaling Technology, Inc.), NADH dehydrogenase (ubiquinone) 1α subcomplex subunit 12 (Ndufa12; cat. no. ab192617; 1:4,000; Abcam), NADH:ubiquinone oxidoreductase subunit AB1 (Ndufab1; cat. no. ab181021; 1:1,000; Abcam), mitochondrial NADH-ubiquinone oxidoreductase chain 1 (MT-ND1; cat. no. ab181848; 1:5,000; Abcam), succinate dehydrogenase (SDHA; cat. no. 11998; 1:4,000; Cell Signaling Technology, Inc.), complex IV (COX IV; cat. no. 11242-1-AP; 1:3,000; Proteintech Group, Inc.), oxoglutarate dehydrogenase (OGDH; cat. no. ab137773; 1:5,000; Abcam), pyruvate dehydrogenase (PDH; cat. no. 3205; 1:3,000; Cell Signaling Technology, Inc.), hypoxia inducible factor-1α (HIF-1α; cat. no. 36169; 1:1,000; Cell Signaling Technology, Inc.), NADPH oxidase 2 (NOX2; cat. no. bs-3889R; 1:1,000; BIOSS), NOX4 (cat. no. bs-1091R; 1:1,000; BIOSS), Bax (cat. no. 2772S; 1:3,000; Cell Signaling Technology, Inc.) and Bcl-2 (cat. no. 3498S; 1:1,000; Cell Signaling Technology, Inc.), GAPDH (cat. no. 10494-1-AP; 1:8,000; Proteintech Group, Inc.), α-tubulin (cat. no. 11224-1-AP; 1:4,000; Proteintech Group, Inc.) and HSP90 (cat. no. 11224-1-AP; 1:2,000; Proteintech Group, Inc.). .. Following incubation with the primary antibody, the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (anti-Mouse secondary antibodies; cat. no. 62-6520; 1:10,000; Thermo Fisher Scientific, Inc. and anti-Rabbit secondary antibodies; cat. no. VJ313046; 1:10,000; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

    Control:

    Article Title: Synergistic protective effects of remote ischemic postconditioning and enalapril on endothelial dysfunction in spontaneously hypertensive rats via inhibition of oxidative stress-mediated mitochondrial dysfunction.
    Article Snippet: .. The primary antibodies used included endothelial NO synthase (eNOS, 32027, clone D9A5L, 0.03 μg/mL, CST, USA), phosphorylated eNOS (p-eNOS, 9570, clone C9C3, 0.2 μg/mL, CST, USA), vascular cell adhesion molecule-1 (VCAM-1, 13662, clone E1E8X, 0.2 μg/mL, CST, USA), intercellular adhesion molecule-1 (ICAM-1, 4915, 0.05 μg/mL, CST, USA), NADPH oxidase (NOX) 2 (ab310337, clone EPR28415-13, 0.5 μg/mL, Abcam), NOX4 (DF6924, 1 μg/mL, Affinity), superoxide dismutase 2 (SOD2, 13141, clone D3X8F, 0.05 μg/mL, CST, USA), mitofusin 2 (MFN2, 9482, clone D2D10, 0.2 μg/mL, CST, USA), optic atrophy 1 (OPA1, 80471, clone D6U6N, 0.03 μg/mL, CST, USA), fission protein 1 (FIS1, 32525, clone E3K9O, 0.03 μg/mL, CST, USA), dynamin-related protein 1 (DRP1, 8570, clone D6C7, 0.4 μg/mL, CST, USA), and β-actin (internal control, 4970, clone 13E5, 0.06 μg/mL, CST, USA). .. After incubation, the membrane was washed and incubated with horseradish peroxidase-conjugated secondary antibodies (7077, 0.1 μg/mL; 7076, 0.2 μg/mL; CST, USA) at room temperature for 1 h. After washin, an enhanced chemiluminescence assay kit (P0018S, Beyotime, China) was added, and the membrane was incubated in the dark for 1 min.



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    Relative protein expression changes to the latent transforming growth factor (TGF)-β–binding protein 4 (LTBP4) pathway and annexin A6 (ANXA6). LTBP4 is a known genetic modifier in human patients with Duchenne muscular dystrophy and impacts TGF-β signaling. TGF-β acts on SMAD4, which directly interacts with mitochondrial cytochrome c oxidase subunit 2 (MTCO2) and can cause mitochondrial fragmentation via dynamin-related <t>protein</t> <t>1</t> <t>(DRP1).</t> A – L: All proteins were assessed for expression levels ( A – F ) relative to total protein ( G – L ). H: TGF-β levels were elevated in D2-WT mice compared with B10-WT mice, and complex IV subunit 2 (MTCO2) was elevated in the D2- mdx mice compared with D2-WT. ∗ P < 0.05, ∗∗ P < 0.01.
    Polyclonal Antibodies Against Dynamin Related Protein 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Relative protein expression changes to the latent transforming growth factor (TGF)-β–binding protein 4 (LTBP4) pathway and annexin A6 (ANXA6). LTBP4 is a known genetic modifier in human patients with Duchenne muscular dystrophy and impacts TGF-β signaling. TGF-β acts on SMAD4, which directly interacts with mitochondrial cytochrome c oxidase subunit 2 (MTCO2) and can cause mitochondrial fragmentation via dynamin-related <t>protein</t> <t>1</t> <t>(DRP1).</t> A – L: All proteins were assessed for expression levels ( A – F ) relative to total protein ( G – L ). H: TGF-β levels were elevated in D2-WT mice compared with B10-WT mice, and complex IV subunit 2 (MTCO2) was elevated in the D2- mdx mice compared with D2-WT. ∗ P < 0.05, ∗∗ P < 0.01.
    Proteintech Dynamin Related Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamin+related+protein+1/DNM3+Antibody/pm41469242-82-49-49
    Average 93 stars, based on 1 article reviews
    proteintech dynamin related protein - by Bioz Stars, 2026-09
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    USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Journal: Military Medical Research

    Article Title: USP18 exacerbates myocardial I/R injury by inhibiting Parkin mitophagy through the deubiquitinase PTEN-L

    doi: 10.1016/j.mmr.2026.100004

    Figure Lengend Snippet: USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Article Snippet: To block mitophagy, the selective dynamin-related protein 1 (Drp1) inhibitor Mdivi-1 was used (50 μmol/L, MedChemExpress, USA).

    Techniques: Inhibition, Electron Microscopy, Transfection, Infection, Ubiquitin Proteomics, Knock-Out

    Relative protein expression changes to the latent transforming growth factor (TGF)-β–binding protein 4 (LTBP4) pathway and annexin A6 (ANXA6). LTBP4 is a known genetic modifier in human patients with Duchenne muscular dystrophy and impacts TGF-β signaling. TGF-β acts on SMAD4, which directly interacts with mitochondrial cytochrome c oxidase subunit 2 (MTCO2) and can cause mitochondrial fragmentation via dynamin-related protein 1 (DRP1). A – L: All proteins were assessed for expression levels ( A – F ) relative to total protein ( G – L ). H: TGF-β levels were elevated in D2-WT mice compared with B10-WT mice, and complex IV subunit 2 (MTCO2) was elevated in the D2- mdx mice compared with D2-WT. ∗ P < 0.05, ∗∗ P < 0.01.

    Journal: The American Journal of Pathology

    Article Title: The D2.B10- Dmd mdx /J Mouse Model of Duchenne Muscular Dystrophy Exhibits a Severe Mitochondrial Deficiency Not Observed in the C57BL/10ScSn- Dmd mdx /J Mouse

    doi: 10.1016/j.ajpath.2025.09.005

    Figure Lengend Snippet: Relative protein expression changes to the latent transforming growth factor (TGF)-β–binding protein 4 (LTBP4) pathway and annexin A6 (ANXA6). LTBP4 is a known genetic modifier in human patients with Duchenne muscular dystrophy and impacts TGF-β signaling. TGF-β acts on SMAD4, which directly interacts with mitochondrial cytochrome c oxidase subunit 2 (MTCO2) and can cause mitochondrial fragmentation via dynamin-related protein 1 (DRP1). A – L: All proteins were assessed for expression levels ( A – F ) relative to total protein ( G – L ). H: TGF-β levels were elevated in D2-WT mice compared with B10-WT mice, and complex IV subunit 2 (MTCO2) was elevated in the D2- mdx mice compared with D2-WT. ∗ P < 0.05, ∗∗ P < 0.01.

    Article Snippet: Antibodies against latent TGF-β–binding protein 4 (LTBP4; Novus Biologicals, Centennial, CO; NBP2-43671; 1:500), TGF-β (Novus Biologicals; NBP2-46108; 1:2000), annexin A6 (ANXA6; ProteinTech, Rosemont, IL; 68086-1-Ig; 1:2000), mitochondrial cytochrome c oxidase subunit 2 (MTCO2; Thermo Fisher; A-6404; 1:500), SMAD4 (ProteinTech; 10231-1-AP; 1:100), dynamin-related protein 1 (DRP1; ProteinTech; 12957-1-AP; 1:1000), and Total OXPHOS Rodent WB Antibody Cocktail (Abcam; ab110413; 1:25,000) were used.

    Techniques: Expressing, Binding Assay